The recent recognition of microarray molecular subtypes points to the need for additional consideration in cell line selection. However, while some investigators choose particular cell lines based on the known ER or HER2 status, many others rely on standard “workhorses” like MCF7 without regard to the particular tumor subtypes being modeled. Advantages of cell lines include the relative ease of pharmacologic and genetic manipulation, the variety of available functional assays, and, for some studies, the purity of the cancerous epithelial population (and absence of stromal cell contamination).

The ERBB2 subtype is associated with expression of genes co-amplified with ERBB2 (encoding HER2) on chromosome cytoband 17q12, and the normal-like subtype shares expression patterns with normal breast tissue.īreast cancer cell lines have been used widely to investigate breast cancer pathobiology, and to screen and characterize new therapeutics. Basal-like tumors share expression markers with the underlying basal (myoepithelial) layer of normal breast ducts, are ER negative, exhibit frequent chromosome segmental gains/losses, and are associated with poor outcome in most studies. 8q24/ MYC), and less favorable prognosis. Luminal-A tumors are genetically simple (1q/16p gain) and are associated with favorable outcome, while luminal-B tumors exhibit high proliferation rates, frequent DNA amplification (e.g.

Luminal subtypes A and B are ER positive and share expression markers with the luminal epithelial layer of cells lining normal breast ducts. More recently, DNA microarray studies have suggested a refined classification of breast cancer, distinguishing five major subtypes based on different patterns of gene expression, underlying DNA copy number alterations (CNAs), and associated clinical outcomes –. Markers such as estrogen receptor (ER), progesterone receptor (PR) and ERBB2/HER2 are used for prognostication, and to stratify patients for appropriately targeted therapies. Breast cancer, a leading cause of cancer death in women, is recognized to be a molecularly heterogeneous disease.

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